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BMG NEPHELOstar Nephelometer

Description

The BMG NEPHELOstar measures intensity of scattered light with an integrating Ulbricht sphere. The instrument can measure 96 and 384 well plates and has an injector to titrate in additional components. Equilibration of samples up to 45 °C is possible.

The nephelometer is highly sensitive to scattered light, and is much more suited than ordinary plate readers to measure turbidity. The increased sensitivity does mean highly scattering samples or plates will give too high background signals for accurate measurements.

Plates

Use the standard polystyrene plates for optimal results. Polypropylene plates are too opaque and give high background scattering.

Solutions

The injector has teflon tubing and a stainless steel needle. Aqueous and polar organic solutions can be titrated.

Titrations

  1. Priming: You must prime the lines before the software will let you do a titration. Make sure to remove the needle from the resting station before you prime the lines, otherwise you will contaminate the measuring chamber of the machine.

Throughput

A simple measurement on a full 96-well plate takes approximately 1 minute. 20 injections in a 96-well plate take approximately 1.5 hour.